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Related Experiment Videos

Microarray expression profiling identifies genes with altered expression in HDL-deficient mice.

M J Callow1, S Dudoit, E L Gong

  • 1Genome Sciences Department, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA. mjcallow@lbl.gov

Genome Research
|December 16, 2000
PubMed
Summary
This summary is machine-generated.

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Altering high-density lipoprotein (HDL) metabolism genes impacts other gene expressions. Researchers identified specific genes affected in HDL-deficient mice using microarray analysis, revealing insights into lipid and oxidative processes.

Area of Science:

  • Genomics
  • Molecular Biology
  • Biochemistry

Background:

  • High-density lipoprotein (HDL) metabolism is crucial for lipid transport.
  • Significant changes in HDL metabolism may influence the expression of other genes.
  • Understanding these interdependencies is key to comprehending broader metabolic regulation.

Purpose of the Study:

  • To identify genes with altered expression in mouse models with significantly reduced HDL plasma concentrations.
  • To investigate the impact of apolipoprotein AI (apoAI) knockout and scavenger receptor BI (SR-BI) overexpression on hepatic gene expression.
  • To explore the utility of microarray analysis coupled with multiple-testing methods for discovering novel gene expression changes.

Main Methods:

  • Screening of over 5000 mouse expressed sequence tags using microarray analysis.

Related Experiment Videos

  • Hybridization of labeled cDNA from the livers of apoAI-knockout mice, SR-BI transgenic mice, and control mice.
  • Application of two-sample t statistics and adjusted P values (< 0.05) to identify significantly altered gene expression.

    • Main Results:

    • In SR-BI transgenic mice, nine array elements (at least five genes) showed significantly altered expression.
    • In apoAI-knockout mice, eight array elements (four genes) exhibited altered expression compared to controls.
    • Genes identified in SR-BI transgenic mice suggest involvement in altered sterol metabolism and oxidative processes.

    Conclusions:

    • Severe alterations in HDL metabolism genes can lead to significant changes in the expression of other genes.
    • Microarray analysis is an effective tool for identifying differentially expressed genes in complex biological systems.
    • The identified genes provide potential targets for further research into HDL metabolism and related pathways.