[Interaction of pancreatic RNAase A with platinum ions]

Complex formation between RNAase and Pt(II) has been studied by gel filtration and conductometry, and spectrophotometry. Existence of three strong binding locations with the binding location of 2 times 10-4 M-1 has been stated. Effect of Pt(II) on hydrolase activity of RNAase Ahas been investigated. An inhibiting effect showed itself at high concentrations of platinum ions. A conclusion has been made concerning the nature of groups participating in complex formation with Pt(II). Modification of three methionin residues does not influence the enzyme activity at 24-48 hour preincubation.