[Typing of isolated mycobacteria]

Microbiological diagnosis of causes of pulmonic or other tuberculosis localization includes microscopic analysis of disease material cause which was taken earlier, his specific elaboration and inoculation at appropriate bases. Bases are incubated until 12 weeks. In the purpose of identification of M. tuberculosis complex or some other (conditionally) potentially pathogenous king of micobacterium, depending on immunity status of the investigated one, it is necessary to apply conventional as well as modern methods of molecular biology in laboratory work. First of all this means differentiation of M. tuberculosis complex from other bacterium (MOT), that is eventually differentiation inside complex. Beside classical tests it is possible to apply some methods of molecular biology in laboratory conditions, as well as nucleus sonds or method for reproduction of one part of nucleus acid which fragments, with a help of restrictive encimes, into several parts (PCR/PRA). With this method it is possible to do complete identification of isolated kinds of micobacterium according to the size of aplicon's fragments (part of reproduces DNA). An appropriate time, which is necessary for execution of these tests, is measured in hours and days, and for the classical tests in weeks and months.