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Molecular cloning and expression profile of Xenopus calcineurin A subunit(1)
T Saneyoshi1, S Kume, T Natsume
1Department of Molecular Neurobiology, Institute of Medical Science, The University of Tokyo, Japan.
Biochimica Et Biophysica Acta
|December 19, 2000
Abstract:
We have cloned a cDNA encoding a catalytic subunit of calcineurin (CnA) expressed in Xenopus oocytes. The deduced amino acid sequence indicates 96.3% and 96.8% identities with the mouse and human CnAalpha isoforms, respectively. Xenopus CnA (XCnA) RNA and protein are expressed as maternal and throughout development. Recombinant XCnA protein interacted with calmodulin in the presence of Ca(2+). Deletion of calmodulin binding domain and auto-inhibitory domain revealed calcium independent phosphatase activity, thereby showing that XCnA is likely to be modulated by both calmodulin and calcium.