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Related Experiment Videos

Some distal limb structures develop in mice lacking Sonic hedgehog signaling.

P Kraus1, D Fraidenraich, C A Loomis

  • 1Ronald O. Perelman Department of Dermatology, New York University School of Medicine, NY, New York, USA.

Mechanisms of Development
|December 19, 2000
PubMed
Summary
This summary is machine-generated.

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Sonic hedgehog (Shh) signaling is crucial for limb development, controlling digit formation and skeletal patterning. Loss of Shh in mice results in distinct digit abnormalities and impacts limb ectoderm outgrowth.

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • Limb patterning involves complex signaling between the apical ectodermal ridge (AER), zone of polarizing activity (ZPA), and limb ectoderm.
  • Interactions include feedback loops involving Sonic hedgehog (Shh), Bone morphogenetic proteins (Bmps), Wnt7a, and Fibroblast growth factors (Fgfs).

Purpose of the Study:

  • To investigate the role of Shh signaling in limb development, particularly digit formation and skeletal patterning in Shh-deficient mice.
  • To clarify the relationship between Shh signaling, Fgf8 expression, and limb outgrowth.

Main Methods:

  • Analysis of Shh-deficient (Shh(-/-)) mouse models to observe limb development.
  • Gene expression analysis, including Shh, Indian hedgehog (Ihh), Fgf8, and Hoxd13.

Related Experiment Videos

  • Morphological assessment of limb structures, including digits, nails, and skeletal elements.

    • Main Results:

    • Shh(-/-) hindlimbs exhibit one distinct digit, while intermediate structures are fused; forelimbs show a less-developed digit and fused bones.
    • Ihh expression is detected in Shh mutant limbs, potentially contributing to distal structure formation.
    • Shh signaling is essential for maintaining Fgf8 expression in the AER, limb outgrowth, and anterior-posterior expansion, with its loss leading to handplate collapse.

    Conclusions:

    • Shh signaling is indispensable for the development of most intermediate hindlimb skeletal elements.
    • Shh regulates AER expansion, zeugopod and autopod branching, and limb ectoderm outgrowth.
    • The study highlights Shh's critical role in establishing distinct limb compartments.