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Gps2, a protein partner for human papillomavirus E6 proteins
Y Y Degenhardt1, S J Silverstein
1Departments of Pharmacology, Columbia University, New York, New York 10032, USA.
Journal of Virology
|December 19, 2000
Summary
Human papillomavirus (HPV) E6 proteins interact with Gps2. High-risk HPV E6 proteins degrade Gps2 and suppress its transcriptional activity, potentially contributing to oncogenesis.
Area of Science:
- Molecular Biology
- Virology
- Cell Biology
Background:
- Human papillomavirus (HPV) is a common viral infection.
- The E6 oncoprotein of HPV plays a critical role in viral oncogenesis.
- Understanding HPV E6 protein interactions is crucial for developing therapeutic strategies.
Purpose of the Study:
- To identify protein partners interacting with human papillomavirus (HPV) E6 proteins.
- To investigate the functional consequences of HPV E6 and Gps2 interaction.
Main Methods:
- Yeast two-hybrid system screening of a human epidermal keratinocyte cDNA library.
- Co-transfection assays and pulse-chase analyses in mammalian cells.
- Assessment of Gps2 transcriptional activation and E6-mediated suppression.
Main Results:
- Identified Gps2 as a protein partner interacting with HPV E6 proteins from both high and low oncogenic risk types.
- Demonstrated that HPV E6 proteins induce the in vivo degradation of Gps2.
- Showed that high-risk HPV E6 suppresses the transcriptional activation activity of Gps2.
Conclusions:
- Gps2 is a novel binding partner of HPV E6 oncoproteins.
- HPV E6-mediated degradation of Gps2 and suppression of its transcriptional activity may contribute to HPV-induced cellular transformation and cancer development.