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Tissue distribution and timing of appearance of polytropic envelope recombinants during infection with SL3-3 murine
K Rulli1, P A Lobelle-Rich, A Trubetskoy
1Department of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, Louisiana 70112, USA.
Abstract:
A time course analysis was performed to identify the sites of formation and timing of appearance of polytropic recombinant viruses following infection of NIH/Swiss mice with the murine retrovirus SL3-3 murine leukemia virus (SL3) or with a weakly pathogenic mutant termed SL3DeltaMyb5. The results indicated that (i) polytropic recombinant viruses occur initially in the thymus of SL3-infected animals, (ii) the timing of appearance of polytropic recombinants in bone marrow is not consistent with their participation in the previously reported formation of transplantable tumor-forming cells at 3 to 4 week postinoculation, and (iii) the efficient generation of recombinant virus is correlated with efficient tumor induction.
Insights
Polytropic recombinant viruses initially form in the thymus after murine leukemia virus infection. Their appearance in bone marrow doesn't align with tumor cell formation, but efficient virus generation correlates with tumor induction.
Area of Science:
- Virology
- Immunology
- Oncology
Background:
- Murine leukemia virus (MLV) infection can lead to the formation of polytropic recombinant viruses.
- Understanding the sites and timing of recombinant virus generation is crucial for elucidating pathogenesis.
Purpose of the Study:
- To determine the primary sites and temporal dynamics of polytropic recombinant virus formation after infection with SL3-3 MLV or its mutant SL3DeltaMyb5.
- To investigate the relationship between recombinant virus generation and tumor induction.
Main Methods:
- Time course analysis in NIH/Swiss mice infected with SL3-3 MLV or SL3DeltaMyb5.
- Identification of polytropic recombinant viruses in thymus and bone marrow.
Main Results:
- Polytropic recombinant viruses were first detected in the thymus of SL3-infected mice.
- The appearance of polytropic recombinants in bone marrow did not correlate with the timing of transplantable tumor-forming cell development.
- Efficient generation of recombinant virus was associated with efficient tumor induction.
Conclusions:
- The thymus is an early site for polytropic recombinant virus formation in SL3-MLV infection.
- Recombinant viruses appearing later in bone marrow may not be directly involved in early tumor formation.
- Recombinant virus replication efficiency is a key factor in MLV-induced tumorigenesis.
