Consensus predictions of membrane protein topology
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Updated: Oct 10, 2026

Determining Membrane Protein Topology Using Fluorescence Protease Protection (FPP)
Published on: April 20, 2015
J Nilsson1, B Persson, G von Heijne
1Stockholm Bioinformatics Center, Department of Biochemistry, Stockholm University, Sweden.
Abstract:
We have explored the possibility that consensus predictions of membrane protein topology might provide a means to estimate the reliability of a predicted topology. Using five current topology prediction methods and a test set of 60 Escherichia coli inner membrane proteins with experimentally determined topologies, we find that prediction performance varies strongly with the number of methods that agree, and that the topology of nearly half of all E. coli inner membrane proteins can be predicted with high reliability (>90% correct predictions) by a simple majority-vote approach.
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