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Related Experiment Videos

Atherosclerosis in apoE knockout mice infected with multiple pathogens.

M S Burnett1, C A Gaydos, G E Madico

  • 1Cardiovascular Research Institute, Washington Hospital Center, Washington, DC, USA.

The Journal of Infectious Diseases
|December 20, 2000
PubMed
Summary

Cytomegalovirus (CMV) and Chlamydia pneumoniae (CP) infection independently worsen atherosclerosis in mice. Co-infection with both pathogens showed a less pronounced increase in lesion size compared to single infections.

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Area of Science:

  • Cardiovascular Research
  • Infectious Disease Immunology
  • Atherosclerosis Pathogenesis

Background:

  • Cytomegalovirus (CMV) and Chlamydia pneumoniae (CP) are suspected contributors to atherosclerosis development.
  • Previous studies indicate that murine CMV (MCMV) and CP can exacerbate lesion size in apolipoprotein E knockout (apoE KO) mice.

Purpose of the Study:

  • To investigate the combined effect of MCMV and CP infections on atherosclerosis lesion size in apoE KO mice.
  • To determine if co-infection with MCMV and CP leads to a greater increase in lesion size than single-pathogen infections.
  • To explore whether MCMV infection alters serum cytokine profiles in a way that promotes lesion development.

Main Methods:

  • Apolipoprotein E knockout (apoE KO) mice were infected with MCMV at 2 weeks of age and CP at 6 and 8 weeks of age.

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  • Control groups received either MCMV alone or CP alone.
  • Mice were euthanized at 16 weeks of age to quantify atherosclerotic lesion areas.

    • Main Results:

    • MCMV infection alone increased lesion size by 84% (P<.001).
    • CP infection alone increased lesion size by 70% (P<.0001).
    • Co-infection with both MCMV and CP resulted in a 45% increase in lesion size (P<.01).

    Conclusions:

    • While both MCMV and CP individually promote atherosclerosis in apoE KO mice, their combined effect on lesion size was less than additive.
    • Elevated circulating interferon-gamma levels following MCMV infection may contribute to increased lesion development.