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Related Concept Videos

Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

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Related Experiment Video

Updated: Jul 22, 2026

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA
13:00

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA

Published on: December 2, 2009

Identifying mRNA subsets in messenger ribonucleoprotein complexes by using cDNA arrays.

S A Tenenbaum1, C C Carson, P J Lager

  • 1Department of Microbiology, Duke University Medical Center, Durham, NC 27710, USA.

Proceedings of the National Academy of Sciences of the United States of America
|December 20, 2000
PubMed
Summary

This study introduces ribonomics, a method using messenger ribonucleoprotein complexes (mRNPs) to identify cell-specific mRNAs. Ribonomics reveals dynamic mRNA clusters involved in posttranscriptional gene regulation.

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Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
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Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

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An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes
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An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes

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Related Experiment Videos

Last Updated: Jul 22, 2026

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA
13:00

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA

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Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
13:34

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Published on: September 29, 2012

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes
09:45

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes

Published on: August 18, 2018

Area of Science:

  • Molecular Biology
  • Genomics
  • Cell Biology

Background:

  • Genomic arrays profile gene expression but struggle to distinguish transcriptional from posttranscriptional regulation.
  • Cell-specific gene expression changes can be obscured in complex tissues like tumors.
  • Messenger ribonucleoprotein complexes (mRNPs) are key players in posttranscriptional gene regulation.

Purpose of the Study:

  • To develop a method for identifying cell type-specific mRNA subsets within mRNPs.
  • To investigate the role of mRNP composition in regulating gene expression.
  • To introduce 'ribonomics' as a novel functional genomics approach.

Main Methods:

  • Utilized cDNA arrays to profile mRNAs within endogenous mRNPs from P19 embryonal carcinoma stem cells.
  • Employed specific mRNA-binding proteins (HuB, eIF-4E, PABP) known to be involved in translation.
  • Analyzed changes in mRNP composition following retinoic acid-induced neuronal differentiation.

Main Results:

  • Identified unique mRNA subsets within specific mRNPs, distinct from total cellular RNA profiles.
  • Observed significant changes in mRNA composition within HuB-mRNP complexes upon neuronal differentiation.
  • Demonstrated that mRNP composition is dynamic and cell type-specific.

Conclusions:

  • The association of mRNAs into mRNPs is dynamic and crucial for posttranscriptional regulation (e.g., mRNA turnover, translation).
  • Ribonomics provides insights into the structural and functional relationships of gene transcripts and protein products.
  • This approach offers a new paradigm for organizing genomic information in a biologically relevant context.