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Related Experiment Videos

Yeast nuclear pore complex assembly defects determined by nuclear envelope reconstruction.

N Gomez-Ospina1, G Morgan, T H Giddings

  • 1Department of Molecular, Cellular and Developmental Biology, CB 347, University of Colorado-Boulder, Boulder, Colorado, 80309-0347, USA.

Journal of Structural Biology
|December 21, 2000
PubMed
Summary

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Nuclear pore complex (NPC) assembly is crucial for cell function. This study shows that mutations in NIC96 and NUP192 genes disrupt NPC assembly in yeast, leading to reduced NPC numbers.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Nuclear pore complexes (NPCs) regulate transport between the nucleus and cytoplasm.
  • NPC assembly is a fundamental cellular process that remains incompletely understood.
  • Identifying genes involved in NPC assembly is key to elucidating this process.

Purpose of the Study:

  • To investigate the role of nucleoporin genes NIC96 and NUP192 in NPC assembly using yeast models.
  • To quantify the impact of mutations in NIC96 and NUP192 on NPC numbers.
  • To establish a functional assay for studying NPC assembly defects.

Main Methods:

  • Utilized conditional alleles of yeast nucleoporin genes NIC96 and NUP192.
  • Employed quantitative analysis of NPC numbers in serially sectioned yeast cells.

Related Experiment Videos

  • Assessed NPC assembly by monitoring NPC counts under restrictive and permissive temperatures.

    • Main Results:

    • Conditional mutations in NIC96 and NUP192 led to a significant decrease in NPC numbers upon temperature shift.
    • The reduction in NPC numbers is attributed to failed assembly of new NPCs during cell division.
    • A subtle defect in NPC numbers was observed in NUP192 mutant cells even at permissive temperatures.

    Conclusions:

    • NIC96 and NUP192 gene products are essential for the proper assembly of nuclear pore complexes.
    • Disruption of these genes impairs the ability of yeast cells to form new NPCs.
    • These findings provide quantitative evidence for the requirement of NIC96 and NUP192 in NPC biogenesis.