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Updated: Aug 19, 2026

Imaging G Protein-coupled Receptor-mediated Chemotaxis and its Signaling Events in Neutrophil-like HL60 Cells
08:24

Imaging G Protein-coupled Receptor-mediated Chemotaxis and its Signaling Events in Neutrophil-like HL60 Cells

Published on: September 14, 2016

MAP kinase upregulation after hematopoietic differentiation: role of chemotaxis

J A Lehman1, C C Paul, M A Baumann

  • 1Department of Physiology and Biophysics, Wright State University School of Medicine, Dayton, Ohio 45435, USA.

American Journal of Physiology. Cell Physiology
|December 21, 2000
PubMed

Insights

Mitogen-activated protein kinase (MAPK) activity decreases during myeloid cell differentiation but increases with maturation. MAPK activation is crucial for neutrophil chemotaxis and response to GM-CSF.

Area of Science:

  • Cell Biology
  • Immunology
  • Signal Transduction

Background:

  • Mitogen-activated protein kinase (MAPK) p42 isoform is active during cell cycle progression.
  • Immature myeloid cells exhibit high basal MAPK activity.
  • Cell differentiation leads to changes in MAPK activity.

Purpose of the Study:

  • To investigate the role of MAPK activity in myeloid cell differentiation and function.
  • To determine the effect of granulocyte/macrophage colony-stimulating factor (GM-CSF) on MAPK activity during neutrophil maturation.
  • To elucidate the involvement of MAPK in neutrophil chemotaxis.

Main Methods:

  • Assessed basal MAPK activity in immature myeloid cell lines (uHL-60, AML-14, MPD).
  • Monitored MAPK activity changes during DMSO-induced differentiation (dHL-60) and in response to GM-CSF.
  • Utilized the MAPK inhibitor PD-98059 to study neutrophil chemotaxis and GM-CSF priming.

Main Results:

  • Basal MAPK activity was high in immature myeloid cells but reduced upon differentiation.
  • GM-CSF induced rapid and robust MAPK activation, with activity increasing with cell maturation.
  • Peripheral blood neutrophils showed the highest MAPK activation (16-fold) in response to GM-CSF.
  • MAPK activation is essential for neutrophil chemotaxis towards interleukin-8 and GM-CSF priming.

Conclusions:

  • MAPK pathway plays a critical role in the terminal differentiation and function of neutrophils.
  • GM-CSF signaling in neutrophils involves MAPK activation for enhanced chemotaxis, independent of proliferation.
  • MAPK activation is a key component of signal transduction pathways regulating mature neutrophil physiology.

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