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Simian virus 40 is present in human lymphomas and normal blood
H David1, S Mendoza, T Konishi
1Division of Hematology/Oncology, Department of Medicine, UCLA School of Medicine, Cedars-Sinai Research Institute, 8700 Beverly Boulevard, Davis 5019, Los Angeles, CA 90048, USA.
Cancer Letters
|December 21, 2000
Summary
Simian virus 40 (SV40) was detected in some blood and lymphoid tissues, but in very low amounts. The findings suggest SV40 presence is adventitious, not directly linked to blood and lymphoid cancers.
Area of Science:
- Virology
- Oncology
- Immunology
Background:
- Simian virus 40 (SV40) has been detected in various human tissues, with some studies suggesting a role in certain cancers.
- The precise role of SV40 in human neoplasia, particularly in blood and lymphoid malignancies, remains largely undetermined.
- Previous research indicates potential oncogenic activity of SV40, necessitating further investigation into its presence in diverse human samples.
Purpose of the Study:
- To determine the prevalence of Simian virus 40 (SV40) in normal and neoplastic blood and lymphoid samples.
- To investigate the potential association of SV40 with lymphomas and other lymphoid-related conditions.
- To analyze the characteristics of detected SV40 sequences in human samples.
Main Methods:
- SV40 presence was assessed in 266 blood and lymphoid samples using amplification followed by hybridization.
- Southern blotting was employed to confirm and quantify viral presence in positive samples.
- Sequence analysis was performed on detected SV40 to compare with known laboratory strains.
Main Results:
- SV40 was detected in 14% of non-AIDS lymphomas, 28% of AIDS-related lymphomas, and 16% of non-cancerous peripheral blood lymphocytes.
- No SV40 was found in leukemia samples.
- Southern blotting indicated viral loads below one genome per ten cells, and sequence analysis revealed SV40 variants distinct from laboratory strains.
Conclusions:
- The limited quantities and distinct sequences of SV40 in blood and lymphoid tissues suggest an adventitious presence.
- SV40 does not appear to have a direct role in the pathogenesis of the studied blood and lymphoid cancers.
- Further research may be needed to elucidate the origins and implications of incidental SV40 detection in human tissues.