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Related Experiment Videos

Early decrease in surface expression of HLA-DQ predicts the development of infection in trauma patients.

J V Taylor1, L E Gordon, H C Polk

  • 1Department of Surgery, Price Institute of Surgical Research, University of Louisville School of Medicine, Louisville, KY 40292, USA.

Clinical and Experimental Immunology
|December 21, 2000
PubMed
Summary
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Human leucocyte antigen-DQ (HLA-DQ) expression on monocytes decreases after trauma. In patients who develop infection, HLA-DQ returns to normal quickly, unlike those without infection, suggesting HLA-DQ is an early outcome marker.

Area of Science:

  • Immunology
  • Trauma research
  • Molecular biology

Background:

  • Human leucocyte antigen-DR (HLA-DR) behavior post-injury is known, but other class II antigens like HLA-DQ are less understood.
  • HLA-DQ genotype may influence responses to bacterial antigens, highlighting its potential role in infection susceptibility.
  • Characterizing HLA-DQ behavior after trauma is crucial for understanding immune responses and patient outcomes.

Purpose of the Study:

  • To analyze and characterize the behavior of human leucocyte antigen-DQ (HLA-DQ) in patients following major trauma.
  • To compare HLA-DQ expression in trauma patients who develop infection versus those who do not.
  • To investigate the potential of HLA-DQ as an early marker for patient outcomes after injury.

Main Methods:

  • Studied 25 patients post-major injury, categorizing them into infected (15) and non-infected (10) groups.

Related Experiment Videos

  • Quantified monocyte HLA-DQ surface expression using FITC-labelled antibodies and flow cytometry.
  • Compared HLA-DQ expression levels with a control group of 11 healthy volunteers.

    • Main Results:

    • Monocyte HLA-DQ expression was reduced in both infected and non-infected trauma patients.
    • HLA-DQ expression returned to normal levels (days 8-14) only in patients who did not develop infection.
    • In patients who later developed infection, monocyte HLA-DQ mean channel fluorescence rapidly normalized post-trauma.
    • Lipopolysaccharide stimulation elevated HLA-DQ expression in infected patients' initial samples, reaching normal ranges.

    Conclusions:

    • Human leucocyte antigen-DQ (HLA-DQ) serves as an additional early marker for patient outcomes following trauma.
    • HLA-DQ's role may extend beyond immune suppression, potentially aiding in self-antigen presentation crucial for initial injury response.
    • Understanding HLA-DQ dynamics post-trauma can offer insights into infection development and host defense mechanisms.