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Related Experiment Videos

Thyroid stimulating hormone increases angiogenic growth factor expression in rat thyrocytes

Cocks1, Ramsden, Watkinson

  • 1(Division of Medical Sciences, Queen Elizabeth Hospital, Edgbaston, Birmingham, UK).

Clinical Otolaryngology and Allied Sciences
|December 21, 2000
PubMed
Summary

Thyroid stimulating hormone (TSH) increases expression of fibroblast growth factor-2 (FGF-2), its receptor (FGFR-1), and Tie-2 in rat thyroid cells. These findings suggest a role for TSH in goitrogenesis-related angiogenesis.

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Area of Science:

  • Endocrinology
  • Molecular Biology
  • Cell Biology

Background:

  • Goitrogenesis involves thyroid stimulating hormone (TSH) receptor hyperstimulation.
  • Angiogenic factors like fibroblast growth factor-2 (FGF-2), FGFR-1, and Tie-2 are elevated in goiters.

Purpose of the Study:

  • Investigate the role of TSH in regulating FGF-2, FGFR-1, and Tie-2 expression in rat thyroid cells (FRTL-5).

Main Methods:

  • FRTL-5 cells were cultured and exposed to varying TSH concentrations.
  • Protein expression of FGF-2, FGFR-1, and Tie-2 was analyzed using Western blotting.

Main Results:

  • TSH elevated FGF-2 expression, peaking at 0.3 mU/ml.
  • TSH dose-dependently increased FGFR-1 fragment expression.

Related Experiment Videos

  • TSH upregulated full-length Tie-2 receptor expression on FRTL-5 cells, mediated by cAMP.

    • Conclusions:

    • Elevated TSH increases FGF-2, FGFR-1, and Tie-2 in rat thyroid cells.
    • These TSH-induced growth factors may drive angiogenesis in goitrogenesis.
    • Targeting these factors could offer new therapeutic strategies for goiter.