Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Differential expression of ovine CD1.

S M Rhind1, J Hopkins, E S Grant

  • 1Department of Veterinary Pathology, Royal (Dick) School of Veterinary Studies, Easter Bush Veterinary Centre, Roslin, Midlothian, UK.

Immunology
|December 21, 2000
PubMed
Summary

This study investigated CD1 molecule expression patterns using molecular techniques. Results show CD1D has the widest expression, unlike the more restricted SCD1B, suggesting distinct roles for CD1 family members.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Challenges of determining the relative contribution of determinants of health on population health: a Canadian perspective.

Perspectives in public health·2024
Same author

Real-world prevalence across 159 872 patients with cancer supports the clinical utility of TMB-H to define metastatic solid tumors for treatment with pembrolizumab.

Annals of oncology : official journal of the European Society for Medical Oncology·2021
Same author

Scrapie infection and endogenous retroviral expression in sheep lymphoid tissues.

Veterinary immunology and immunopathology·2021
Same author

Variation in zoobenthic blue carbon in the Arctic's Barents Sea shelf sediments.

Philosophical transactions. Series A, Mathematical, physical, and engineering sciences·2020
Same author

Burkholderia pseudomallei multi-centre study to establish EUCAST MIC and zone diameter distributions and epidemiological cut-off values.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2020
Same author

An open and closed case: timing of closure following laparostomy.

Annals of the Royal College of Surgeons of England·2020

Area of Science:

  • Immunology
  • Molecular Biology

Background:

  • CD1 molecules are crucial for antigen presentation.
  • Previous studies suggest varied expression patterns of CD1 family members across species.
  • Understanding CD1 expression is key to elucidating immune responses.

Purpose of the Study:

  • To determine the molecular-level expression patterns of CD1 molecules.
  • To differentiate expression profiles of SCD1B and SCD1D.
  • To address discrepancies in CD1 expression data from prior research.

Main Methods:

  • Utilized reverse transcription-polymerase chain reaction (RT-PCR) with specific primers.
  • Employed Northern and in situ hybridization techniques.
  • Used a pan-CD1 probe (exon 4) and isotype-specific probes for SCD1B and SCD1D.

Related Experiment Videos

Main Results:

  • Widespread CD1 expression detected in thymus, lymphocytes, lung, and intestine using pan-CD1 and SCD1D probes.
  • SCD1B expression was restricted to the thymus and dendritic cells.
  • Highlighted differential expression between CD1 group 1 and group 2 members.

Conclusions:

  • SCD1D exhibits the broadest expression pattern among CD1 family members.
  • SCD1B shows a more restricted expression profile.
  • Differential expression suggests distinct functional roles for various CD1 family members in immunity.