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Related Experiment Videos

Fiber-optic immunosensor for mycotoxins.

C M Maragos1, V S Thompson

  • 1National Center for Agricultural Utilization Research, Peoria, Illinois 61604, USA.

Natural Toxins
|December 21, 2000
PubMed
Summary
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Evanescent wave fiber-optic immunosensors effectively detect fumonisins and aflatoxins in maize. Sample clean-up significantly impacts detection limits, with affinity columns improving fumonisin detection by tenfold.

Area of Science:

  • Analytical Chemistry
  • Biosensing Technology
  • Food Safety

Background:

  • Mycotoxin contamination, particularly fumonisins and aflatoxins, poses a significant threat to maize quality and human health.
  • Accurate and rapid detection methods are crucial for ensuring food safety and agricultural trade.
  • Fiber-optic immunosensors offer a promising platform for sensitive and selective toxin detection.

Purpose of the Study:

  • To develop and evaluate evanescent wave-based fiber-optic immunosensors for detecting fumonisin B1 (FB1) and aflatoxin B1 (AFB1) in maize.
  • To compare the performance of competitive and non-competitive assay formats.
  • To assess the impact of sample pre-treatment methods on sensor sensitivity and accuracy.

Main Methods:

  • Development of competitive fiber-optic immunosensors for FB1 using monoclonal antibodies and fluorescence labeling.

Related Experiment Videos

  • Development of a non-competitive fluorescence-based sensor for AFB1 detection.
  • Evaluation of different maize sample clean-up procedures (dilution vs. affinity column).
  • Comparison of immunosensor results with High-Performance Liquid Chromatography (HPLC) for FB1 detection.

    • Main Results:

    • The competitive FB1 immunosensor showed an inverse relationship between signal and toxin concentration.
    • Affinity column clean-up improved FB1 detection limits by tenfold compared to simple dilution.
    • The non-competitive AFB1 sensor detected toxins directly via native fluorescence, with a limit of detection of 2 ng/mL.
    • Immunosensor and HPLC methods showed good agreement for FB1, with discrepancies arising from cross-reactive fumonisins.

    Conclusions:

    • Evanescent wave fiber-optic sensors demonstrate potential for rapid screening of mycotoxins in maize.
    • Effective sample clean-up is essential for optimizing the performance of these immunosensors.
    • Further development may lead to robust tools for real-time food safety monitoring.