Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Conformational changes in the ribosome induced by translational miscoding agents.

O Jerinic1, S Joseph

  • 1Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0314, USA.

Journal of Molecular Biology
|December 22, 2000
PubMed
Summary

Translational miscoding agents like aminoglycosides alter ribosome structure, impacting protein synthesis accuracy. This study confirms these structural changes correlate with their effects in living organisms.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical value of anti-desmoglein-2 antibodies in arrhythmogenic right ventricular cardiomyopathy: real-world evidence.

Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology·2026
Same author

In vitro evaluation of anti-inflammatory, anti-oxidant activity of pomegranate peel extract mediated calcium sulfate nano particles.

The Medical journal of Malaysia·2025
Same author

An in vitro evaluation of anti-oxidant properties of novel nano-composite material containing titanium oxide, zinc oxide and green tea extract.

The Medical journal of Malaysia·2025
Same author

Substrate stiffness promotes vascular smooth muscle cell calcification by reducing the levels of nuclear actin monomers.

Journal of molecular and cellular cardiology·2024
Same author

Reporting of paediatric osteoporotic vertebral fractures in Duchenne muscular dystrophy and potential impact on clinical management: the need for standardised and structured reporting.

Pediatric radiology·2023
Same author

Endocrine and Bone Monitoring in Boys with Duchenne Muscular Dystrophy; Do we adhere to the standards of care?

Journal of neuromuscular diseases·2023

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Ribosomes translate mRNA to proteins, a crucial process for life.
  • Translational fidelity is vital; miscoding agents compromise this accuracy.
  • Aminoglycosides are miscoding agents known to affect ribosome structure and translation.

Purpose of the Study:

  • To investigate how miscoding agents induce conformational changes in Escherichia coli ribosomes.
  • To correlate in vitro observed structural changes with in vivo phenotypes.

Main Methods:

  • Utilized a toeprinting assay to detect structural alterations in ribosomes.
  • Tested various aminoglycosides (streptomycin, neomycin, kanamycin, gentamycin, hygromycin B), miscoding agents (viomycin, ethanol), and non-miscoding antibiotics (tetracycline, chloramphenicol, erythromycin, fusidic acid, spectinomycin).

Related Experiment Videos

  • Examined ribosomes from streptomycin-resistant and streptomycin-dependent strains.

    • Main Results:

    • Streptomycin, neomycin, kanamycin, gentamycin, and hygromycin B induced conformational changes in E. coli ribosomes.
    • Other miscoding agents (viomycin, ethanol) also caused similar structural changes.
    • Non-miscoding antibiotics did not induce these conformational changes.
    • Ribosomes from streptomycin-resistant or dependent strains did not exhibit changes with streptomycin.

    Conclusions:

    • Miscoding agents trigger specific conformational changes in bacterial ribosomes.
    • These structural alterations are linked to the observed in vivo effects of miscoding agents.
    • The findings provide a molecular basis for understanding aminoglycoside action.