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Hemodialysis does not influence the peroxidative state already present in uremia
A Hirayama1, S Nagase, M Gotoh
1Department of Internal Medicine, Institute of Clinical Medicine, University of Tsukuba, Japan. aki-hira@md.tsukuba.ac.jp
Nephron
|December 22, 2000
Summary
Hemodialysis patients exhibit elevated lipid peroxidation in plasma and erythrocytes. This oxidative stress is linked to end-stage renal disease, not the hemodialysis procedure itself.
Area of Science:
- Nephrology
- Biochemistry
- Oxidative Stress Research
Background:
- Patients undergoing hemodialysis (HD) experience significant oxidative stress.
- The precise nature and origin of this oxidative stress in HD patients remain unclear.
Purpose of the Study:
- To investigate the role of phosphatidylcholine hydroperoxide (PCOOH) as a marker of lipid peroxidation in hemodialysis patients.
- To evaluate the impact of end-stage renal disease and the hemodialysis procedure on oxidative stress markers.
Main Methods:
- Measured plasma and erythrocyte membrane thiobarbituric acid reactive substances (TBARS) and PCOOH levels.
- Assessed TBARS and PCOOH in HD patients' plasma before and after dialysis sessions.
- Compared levels in HD patients with healthy controls.
Main Results:
- HD patients showed significantly higher plasma TBARS and PCOOH levels compared to controls, both before and after dialysis.
- Erythrocyte PCOOH levels were also significantly elevated in HD patients.
- Plasma PCOOH levels decreased post-dialysis but remained higher than in controls.
- Polycystic kidney disease patients had lower plasma PCOOH than other HD patient groups.
Conclusions:
- Hemodialysis patients exhibit increased lipid peroxidation in plasma and erythrocytes, indicative of heightened oxidative stress.
- The observed oxidative stress is primarily associated with end-stage renal disease, not directly caused by the hemodialysis treatment.