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REBASE--restriction enzymes and methylases.

R J Roberts1, D Macelis

  • 1New England BioLabs, 32 Tozer Road, Beverly, MA 01915, USA. roberts@neb.com

Nucleic Acids Research
|January 11, 2000
PubMed
Summary

REBASE is a comprehensive database for restriction enzymes and related proteins, offering detailed data on recognition sites, methylation sensitivity, and genomic analyses. It provides crucial information for molecular biology research and applications.

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Area of Science:

  • Molecular Biology
  • Genomics
  • Bioinformatics

Background:

  • Restriction enzymes and DNA methylases are essential tools in molecular biology.
  • Databases are crucial for organizing and accessing information on these enzymes.
  • REBASE has historically served as a key resource for restriction enzyme data.

Purpose of the Study:

  • To provide a comprehensive and updated resource for restriction enzymes, DNA methylases, and related proteins.
  • To integrate new data, including methylation sensitivity and genomic analyses.
  • To ensure accessibility of this vital data to the scientific community.

Main Methods:

  • Data compilation from published and unpublished literature.
  • Inclusion of recognition and cleavage sites, isoschizomers, and commercial availability.
  • Analysis of restriction systems in sequenced bacterial and archaeal genomes.

Main Results:

  • REBASE contains extensive information on restriction enzymes, DNA methylases, nicking enzymes, and associated proteins.
  • New features include detailed methylation sensitivity data and analyses of genomic restriction systems.
  • Data is accessible via email, FTP, and the World Wide Web.

Conclusions:

  • REBASE remains an indispensable, continuously updated resource for researchers in molecular biology and genomics.
  • The database facilitates research by providing consolidated information on enzyme properties and genomic contexts.
  • Enhanced features improve the utility of REBASE for understanding and utilizing restriction-modification systems.

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