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Updated: Oct 10, 2026

A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells
07:09

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Published on: July 31, 2012

Interleukin-12 induces gene expression in interleukin-2 stimulated human T lymphocytes

B Li1, D Cao, H Xu

  • 1Department of Immunology, Harbin Medical University, Harbin 150086, PR China.

European Cytokine Network
|December 23, 2000
PubMed

IL-12 is a critical immunoregulatory cytokine that promotes cell-mediated immune responses by inducing the differentiation of Th1 cells. To better clarify the molecular basis of IL-12 action, we compared the gene expression in human T lymphocytes activated by IL-2 and IL-12. mRNAs from T lymphocytes activated by either IL-2 alone or IL-2 plus IL-12 were transcribed into cDNAs. A differential mRNA display was conducted. As a result, differential display of five cDNA fragments was obtained. Sequence analysis suggests that they had high homology with recorded genes as found by a computer search against GenBank. Two full genes of the five fragments were cloned, which activation-induced C-type lectin and glucose transporter-like protein. Interestingly, these proteins were expressed in the T cells stimulated by IL-2 and IL-12, but not in the T cells stimulated by IL-2 alone. These results suggest that C-type lectin and glucose transporter-like protein may play an important role in the T lymphocyte activation induced by IL-12.

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