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From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia
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Myeloma proteins that bind Hsp65 (GroEL) are polyreactive and are found in high incidence in pristine induced

M Potter1, G Jones, W Dubois

  • 1Laboratory of Genetics, National Cancer Institute, National Institute of Health, Bethesda, MD 20892 USA

Current Topics in Microbiology and Immunology
|December 28, 2000
PubMed

The myeloma proteins produced by 44 plasmacytomas (PCTs) recently induced by pristane in BALB/cAnPt and closely related PCT susceptible congenic strains of mice were isolated chromatographically and screened against a panel of 10 protein, nucleic acid and lipid antigens. This sample was highly unusual because 82% of the proteins had IgG isotopes. Nine of the proteins bound to Hsp65 (GroEL), and all of these were polyreactative. Twenty-one of the myeloma proteins were polyreactive and bound two or more antigens in the panel, and five were monoreactive. Thus, an antigen binding activity was determined for 59% of these myeloma proteins.

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