Nitrous oxide, but not xenon, affects the signaling in the neuronal growth cone

1. Xenon (Xe) is an inert gas with the anesthetic property. To investigate whether Xe affects the neural network formation, the authors examined the biochemical characteristics of growth cones prepared from rat forebrains at different perinatal periods, in comparison with inhalation of N2O. 2. Fetal or neonatal rats were exposed to an atmosphere containing inhalational anesthetics (70% Xe or N2O) or the control atmosphere (30% O2 and 70% N2) for 6 h. After the exposure, isolated growth cone particles (IGCs) were prepared from their forebrains using a subcellular fractionation method. Protein composition, Ca2+-dependent protein phosphorylation, protein kinase C (PKC) activity, and degradation of PKC in the IGCs were compared among three groups. 3. No apparent change of protein composition in IGCs was observed by electrophoresis. Ca2+dependent phosphorylation of GAP-43 and MARCKS protein, and PKC activity in IGCs significantly decreased after exposure to N20. The degradation of PKC increased significantly after inhalation of N2O. 4. The authors concluded that Xe dose not change the above biochemical characteristic of the growth cones, suggesting that Xe is free from the teratogenic effect on the neuronal network formation and that Xe is a safe anesthetics for the perinatal neuronal development.