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Protective effects of manganese against lipid peroxidation
1Graduate Institute of Medicine and School of Technology for Medicine Sciences, Kaohsiung Medical University, Taiwan, Republic of China.
Abstract:
The aim of this study was to investigate the effects of chronic, daily, 30-d administration of manganese chloride (MnCl2) to male Sprague-Dawley rats on lipid peroxidation in various tissues. Rats were intraperitoneally injected with MnCl2 (20 mg/kg) once daily for 30 consecutive days. The Mn accumulated in liver, spleen, adrenal glands, heart, kidneys, lung, and testes. This was associated with decreased lipid peroxidation in liver, spleen, and adrenal glands and a decrease in the levels of Fe in these tissues. In a second group of animals, Mn (20 mg/kg/d) and glutathione (GSH, 15 mg/kg/d) were administered ip for 30 d. GSH counteracted the Mn-induced protective fall in lipid peroxidation, but Fe levels remained lower in liver and spleen. Mn decreases lipid peroxidation in certain tissues, which may involve lowering Fe content, but interaction with Fe is not the sole mechanism.
Insights
Chronic manganese chloride exposure in rats reduced lipid peroxidation in several organs. Glutathione administration partially reversed this effect, indicating manganese
Area of Science:
- Toxicology
- Biochemistry
- Trace Element Research
Background:
- Manganese (Mn) is an essential trace element with complex biological roles.
- Chronic exposure to manganese chloride (MnCl2) can lead to toxicity.
- The impact of Mn on lipid peroxidation, a marker of oxidative stress, requires further investigation.
Purpose of the Study:
- To examine the effects of chronic MnCl2 administration on lipid peroxidation in rat tissues.
- To determine if manganese accumulation correlates with changes in lipid peroxidation.
- To investigate the role of glutathione (GSH) in modulating Mn-induced effects on lipid peroxidation.
Main Methods:
- Male Sprague-Dawley rats received daily intraperitoneal injections of MnCl2 (20 mg/kg) for 30 days.
- Tissue samples were analyzed for manganese content and lipid peroxidation levels.
- A subset of rats received co-administration of MnCl2 and GSH (15 mg/kg/d).
Main Results:
- Manganese accumulated in the liver, spleen, adrenal glands, heart, kidneys, lung, and testes.
- MnCl2 administration decreased lipid peroxidation and iron (Fe) levels in the liver, spleen, and adrenal glands.
- GSH partially reversed the Mn-induced decrease in lipid peroxidation, but Fe levels remained reduced in the liver and spleen.
Conclusions:
- Chronic manganese exposure decreases lipid peroxidation in specific tissues, potentially through mechanisms involving iron reduction.
- The interaction between manganese and iron is implicated but not the sole factor in Mn-induced changes in lipid peroxidation.
- Glutathione can counteract some of the effects of manganese on lipid peroxidation.