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Determination of domoic acid in Japanese mussels by enzyme immunoassay

K Kawatsu1, Y Hamano, T Noguchi

  • 1Osaka Prefectural Institute of Public Health, Department of Food Microbiology, Nakamichi, Japan.

Journal of AOAC International
|December 29, 2000
PubMed

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.