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Published on: April 5, 2019
Selectivity in microemulsion electrokinetic chromatography
S Pedersen-Bjergaard1, C Gabel-Jensen, S Honoré Hansen
1Department of Analytical and Pharmaceutical Chemistry, Royal Danish School of Pharmacy, Copenhagen.
Journal of Chromatography. A
|December 29, 2000
Summary
Microemulsion electrokinetic chromatography (MEEKC) offers efficient separation of various solutes. Adjusting surfactant type, pH, and temperature are key to optimizing selectivity in MEEKC separations.
Area of Science:
- Analytical Chemistry
- Separation Science
Background:
- Microemulsion electrokinetic chromatography (MEEKC) is a powerful separation technique.
- It offers high efficiency and selectivity for anionic, cationic, and neutral compounds.
Purpose of the Study:
- To investigate methods for altering selectivity in MEEKC.
- To identify key factors influencing solute separation in MEEKC.
Main Methods:
- MEEKC utilizing microemulsions as pseudo-stationary phases.
- Systematic variation of surfactant type, pH, and temperature.
Main Results:
- Selectivity in MEEKC can be effectively tuned by changing the surfactant composition.
- pH adjustments significantly impact separation selectivity.
- Temperature variations also influence the selectivity of the MEEKC system.
- The nature of the organic phase has minimal impact on the separation of lipophilic solutes.
Conclusions:
- MEEKC provides a versatile platform for chromatographic separations.
- Surfactant choice, pH, and temperature are critical parameters for optimizing MEEKC selectivity.
- These findings enable tailored separations for diverse analytical challenges.
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