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In vitro selection of hematoporphyrin binding DNA aptamers
A Okazawa1, H Maeda, E Fukusaki
1Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Japan.
Bioorganic & Medicinal Chemistry Letters
|December 29, 2000
Abstract:
DNA aptamers that bind to hematoporphyrin IX (HPIX) were isolated using an in vitro selection technique. Most aptamers obtained after the 7th and 10th rounds contained guanine-rich sequences. Binding assay using fluorescence polarization technique and structural analysis by CD spectra revealed that the parallel guanine-quartet structure of the aptamer participates in the recognition of HPIX.