Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Low glucocorticoid concentrations decrease oxidative phosphorylation of isolated rat brain mitochondria: an

C Morin1, R Zini, N Simon

  • 1Département de Pharmacologie, Faculté de Médecine de Paris XII, Créteil, France. tillement@univ-paris12.fr

Fundamental & Clinical Pharmacology
|December 29, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Practical implementation of automated insulin delivery systems in 2025: A French position statement update.

Diabetes & metabolism·2025
Same author

Evaluation of polybrominated diphenyl ether (PBDE) flame retardants from various materials in professional seating furnishing wastes from French flows.

Waste management (New York, N.Y.)·2021
Same author

Ischemic cerebrovascular diseases in patients with COVID-19.

Revue neurologique·2020
Same author

[Splenic infarction revealing Plasmodium vivax acute malaria].

Medecine et maladies infectieuses·2020
Same author

Corticosterone mediates telomere length in raptor chicks exposed to chemical mixture.

The Science of the total environment·2019
Same author

Bioaccessibility of polycyclic aromatic compounds (PAHs, PCBs) and trace elements: Influencing factors and determination in a river sediment core.

Journal of hazardous materials·2019
Same journal

The Challenges of Scientific Publishing: An Editor-In-Chief's Perspective.

Fundamental & clinical pharmacology·2026
Same journal

Glucagon-Like Peptide-1 Receptor Agonists in Cocaine Use Disorder: Clinical Observations and Underlying Mechanisms.

Fundamental & clinical pharmacology·2026
Same journal

TAAR1-Associated Trace Amines for Cutaneous Nociceptive Blockade in Rats.

Fundamental & clinical pharmacology·2026
Same journal

Synergistic Cardioprotection of Colchicine and Hyperbaric Oxygen Therapy in a Rat Model of Remote Ischemia-Reperfusion Injury.

Fundamental & clinical pharmacology·2026
Same journal

Probucol Exerts Anticancer Effects on SW480 Human Colorectal Cancer Cells via Modulation of Apoptosis- and Angiogenesis-Related Proteins.

Fundamental & clinical pharmacology·2026
Same journal

Nebivolol Protects the Thoracic and Abdominal Aorta and Their Perivascular Adipose Tissue From the Differential Detrimental Effects of Obesity.

Fundamental & clinical pharmacology·2026
See all related articles

Glucocorticoids like dexamethasone inhibit mitochondrial function by affecting oxidative phosphorylation and ATP synthesis at low concentrations. Dexamethasone uniquely impacts complex I and reduces damaging superoxide anion generation.

Area of Science:

  • Biochemistry
  • Neuroscience
  • Pharmacology

Background:

  • Mitochondria are crucial for cellular energy production and are implicated in various pathophysiological processes.
  • Glucocorticoids are widely used for their anti-inflammatory and immunosuppressive effects, but their impact on cellular energetics is not fully understood.

Purpose of the Study:

  • To investigate the in vitro effects of four glucocorticoids (hydrocortisone, triamcinolone, prednisolone, dexamethasone) on rat brain mitochondria.
  • To assess the impact of these drugs on mitochondrial respiration, ATP synthesis, enzyme activities, and reactive oxygen species generation.

Main Methods:

  • Isolation of mitochondria from rat brain.
  • Measurement of respiratory control ratio (RCR), oxygen consumption, and ATP synthesis.

Related Experiment Videos

  • Assay of enzymatic activities of mitochondrial complexes and superoxide anion generation.

    • Main Results:

    • All tested glucocorticoids decreased RCR by inhibiting oxidative phosphorylation (State 3) and Complex V activity, altering mitochondrial inner membrane proton fluxes at sub-nanomolar concentrations.
    • Dexamethasone specifically inhibited Complex I activity and reduced superoxide anion radical generation.
    • Inhibition of Complex V and partial uncoupling reversal were common to all tested glucocorticoids.

    Conclusions:

    • Glucocorticoids can modulate mitochondrial function, oxygen consumption, ATP synthesis, and superoxide production.
    • These effects, particularly those of dexamethasone on Complex I and superoxide generation, may have significant implications in various pathophysiological conditions.
    • The findings highlight a direct impact of glucocorticoids on mitochondrial bioenergetics at pharmacologically relevant concentrations.