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Transgenic animal bioreactors.

L M Houdebine1

  • 1Unite de Biologie du Développement et Biotechnologie, Institut National de la Recherche Agronomique, Jouy-en-Josas, France. houdebine@biotec.jouy.inra.fr

Transgenic Research
|December 29, 2000
PubMed
Summary

Transgenic animals offer a scalable method for producing complex recombinant proteins, but challenges in gene expression and post-translational modifications require further optimization for industrial application.

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Area of Science:

  • Biotechnology
  • Genetic Engineering
  • Animal Science

Background:

  • Recombinant protein production is a key biotechnological achievement.
  • Transgenic animals are utilized for synthesizing proteins with crucial post-translational modifications.
  • Various biological sources from transgenic animals are explored for industrial-scale recombinant protein production.

Purpose of the Study:

  • To review the progress and challenges in using transgenic animals for recombinant protein production.
  • To identify technical hurdles in optimizing expression systems and ensuring proper protein modification.
  • To discuss strategies for improving transgene expression and mitigating potential side effects.

Main Methods:

  • Utilizing transgenic animal models for pharmaceutical protein production.
  • Employing advanced techniques like animal cloning and YAC/BAC vectors for gene delivery.
  • Investigating gene regulatory elements (insulators, enhancers, introns) to enhance transgene expression.
  • Implementing inducible expression systems for controlled protein production.
  • Developing sensitive tests for biological contaminant screening, such as prions.

Main Results:

  • Successful production of complex recombinant proteins at industrially relevant levels.
  • Demonstration of enhanced protein maturation through the co-expression of specific enzymes (e.g., Furin).
  • Identification of strategies to minimize deleterious effects of recombinant proteins on host animals.
  • Development of purification protocols for recombinant proteins from various biological fluids.

Conclusions:

  • Transgenic animal technology holds significant promise for industrial recombinant protein production.
  • Continued research into gene expression regulation and protein modification is essential for system optimization.
  • Addressing challenges related to cost, efficiency, and safety is crucial for the widespread adoption of this technology.
  • Potential applications extend to improving animal product composition and health.

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