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Specific binding of IL-8 to rabbit alpha-macroglobulin modulates IL-8 function in the lung
A Kurdowska1, N Fujisawa, B Peterson
1Department of Biochemistry, University of Texas Health Center, Tyler 75708, USA. anna.kurdowska@uthct.edu
Objective And Design:
The purpose of this study was to compare chemotactic activity of IL-8 alone with that of IL-8 reacted with rabbit alpha-macroglobulins (alphaM) in vivo.
Methods:
Initially the binding of recombinant human IL-8 (rhIL-8) to rabbit alphaM was studied. 125I-labeled rhIL-8 was incubated with alphaM, and electrophoresed on native 5% gels or SDS-polyacrylamide 4-20% gradient gels. Next, rhIL-8 or rhIL-8 bound to alphaM was administered via an endotracheal tube to rabbit's lungs.
Treatment:
An endotracheal tube was wedged into a segment of the lobe of each lung, and a sample instilled through the tube into this segment. After 4 h the lungs were lavaged.
Results:
rhIL-8 bound to alphaM retained its full chemotactic activity in vitro but exhibited a diminished ability to induce the influx of neutrophils into the rabbit lung.
Conclusions:
The data suggest that alphaM may facilitate IL-8 clearance from the lung.