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Mean platelet volume is a useful parameter: a reproducible routine method using a modified Coulter thrombocytometer
A J Bancroft1, E W Abel, M Mclaren
1The University Department of Medicine, Ninewells Hospital and Medical School, Dundee, UK. a.j.bancroft@dundee.ac.uk
Platelets
|December 29, 2000
Summary
This study introduces a new, reliable instrument for measuring mean platelet volume (MPV), a key indicator of platelet activity. The findings establish normal MPV ranges and reveal its relationship with age and platelet count.
Area of Science:
- Hematology
- Biomedical Engineering
Background:
- Platelets play a crucial role in hemostasis, thrombosis, and atherogenesis.
- Platelet volume, particularly mean platelet volume (MPV), is a significant marker of platelet function and activation.
- Larger platelets are generally more active, making MPV a determinant of platelet behavior.
Purpose of the Study:
- To develop a sensitive and reliable instrument for measuring platelet volume.
- To establish a reproducible method for assaying MPV, median (MED), and skewness (SK).
- To investigate the influence of anticoagulants and assay timing on MPV measurements.
Main Methods:
- Development of a novel instrument using a computer-interfaced Coulter Thrombocytometer and a high-speed pulse analyzer.
- Establishment of a reproducible assay for MPV, MED, and SK derived from platelet volume histograms.
- Analysis of factors including anticoagulant type and time elapsed before assay.
Main Results:
- A normal range for MPV was established, showing direct correlation with MED and inverse correlation with SK.
- MPV was found to decrease with age, with no significant gender-based differences.
- A negative correlation was observed between whole blood platelet count and MPV/MED, and a direct relationship with SK.
Conclusions:
- The developed instrument provides a reliable and specific method for assessing platelet volume.
- MPV is a valuable parameter influenced by age and platelet production rate.
- Understanding MPV dynamics is crucial for evaluating platelet function in various physiological and pathological conditions.