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[Construction of the physical map of Pi-2(t), a blast resistance gene in rice]
1Institute of Genetics, College of Life Sciences, Wuhan University, Wuhan 430072, China.
Abstract:
In an effort to clone a rice blast resistance gene, Pi-2(t), a BAC contig consisting of 22 BAC clones covering the whole Pi-2(t) region, was constructed using marker-based chromosome landing and chromosome walking. The genetic span of this physical map is 8 cM, but the physical size is 925 kb. The physical map forms the base of further isolation and cloning of Pi-2(t) gene, it will provide new markers for molecular marker-aided selection of new blast resistance materials in rice.
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