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[Pseudomonas aeruginosa].

M Aihara1, M Komatsu

  • 1Department of Microbiology, Tenri Hospital, Tenri 632-8552.

Rinsho Byori. the Japanese Journal of Clinical Pathology
|January 2, 2001
PubMed
Summary
This summary is machine-generated.

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Contaminated nasal feeding solutions likely caused a multi-drug resistant Pseudomonas aeruginosa outbreak in a hospital's internal medicine ward. This resistant bacteria strain led to infections in 40% of patients, primarily those with brain-vesicular damage.

Area of Science:

  • Infectious Diseases
  • Microbiology
  • Hospital Epidemiology

Background:

  • Nosocomial infections caused by multi-drug resistant Pseudomonas aeruginosa (MDRPS) pose a significant threat in healthcare settings.
  • An outbreak of MDRPS occurred in the Internal Medicine Ward E of a hospital in 1999, affecting 50 patients.

Purpose of the Study:

  • To investigate the source and transmission routes of the MDRPS outbreak.
  • To identify risk factors associated with MDRPS infection in the affected patient population.

Main Methods:

  • Isolation and identification of MDRPS from patient samples (sputum, urine, wound discharge, catheter).
  • Antimicrobial susceptibility testing, including minimum inhibitory concentration (MIC) for imipenem.
  • Environmental sampling of equipment, wastewater, and nutrient feeding solutions.

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  • Molecular typing using random amplified polymorphic DNA (RAPD) PCR to assess DNA homology.

    • Main Results:

    • MDRPS was isolated from 20 (40.0%) patients, with resistance to multiple antibiotics including piperacillin, ceftazidime, sulbactam/cefoperazone, minocycline, and ofloxacin.
    • 40% of isolates showed an imipenem MIC of >8 µg/ml.
    • MDRPS was detected in nutrient fluid for nasal feeding and in the feeding line of one patient, but not in general equipment or wastewater.
    • RAPD PCR confirmed DNA homology between MDRPS strains from patients and the contaminated nutrient fluid.

    Conclusions:

    • Contaminated nutrient fluid used for nasal feeding is a likely source of the MDRPS outbreak.
    • This highlights the critical importance of strict hygiene protocols in managing medical feeding supplies to prevent nosocomial infections.