Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Differential gene expression of hepatocellular carcinoma using cDNA microarray analysis.

W Y Lau1, P B Lai, M F Leung

  • 1Department of Surgery, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, SAR. josephlau@cuhk.edu.hk

Oncology Research
|January 2, 2001
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparison of the mechanical properties of different skin sites for auricular and nasal reconstruction.

Journal of otolaryngology - head & neck surgery = Le Journal d'oto-rhino-laryngologie et de chirurgie cervico-faciale·2017
Same author

Reducing unnecessary preoperative testing in elective ENT surgery: clinical and financial implications.

Journal of perioperative practice·2016
Same author

A case of chemical assault in Hong Kong (case report).

International journal of surgery case reports·2015
Same author

Transcriptional silencing of the TMS1/ASC tumour suppressor gene by an epigenetic mechanism in hepatocellular carcinoma cells.

The Journal of pathology·2007
Same author

Wilson's disease with chronic active hepatitis: monitoring by in vivo 31-phosphorus MR spectroscopy before and after medical treatment.

AJR. American journal of roentgenology·2004
Same author

In vitro cytotoxicity testing of a nanocrystalline silver dressing (Acticoat) on cultured keratinocytes.

British journal of biomedical science·2004

This study used cDNA microarray to analyze gene expression in hepatocellular carcinoma (HCC) tissues. Researchers identified differentially expressed genes in HCC, finding it an effective method for detection.

Area of Science:

  • Molecular Biology
  • Genomics
  • Oncology

Background:

  • Hepatocellular carcinoma (HCC) is a significant global health concern.
  • Understanding differential gene expression in HCC is crucial for diagnosis and treatment.
  • Previous methods for gene expression analysis have limitations.

Purpose of the Study:

  • To investigate differential gene expression in HCC using cDNA microarray technology.
  • To identify specific genes that are upregulated or downregulated in HCC tissues compared to non-tumorous tissues.
  • To evaluate the efficacy of cDNA microarray for HCC gene expression profiling.

Main Methods:

  • Utilized a cDNA microarray technique for simultaneous analysis of over 4000 human genes.
  • Analyzed gene expression in 10 pairs of HCC and adjacent non-tumorous tissues from Hong Kong Chinese patients.

Related Experiment Videos

  • Employed two-tailed Wilcoxon test for statistical significance (P < 0.05).

    • Main Results:

    • Identified 211 highly expressed and 147 downregulated genes in over 10% of HCC pairs.
    • Found 6 highly expressed and 10 downregulated genes in over 30% of HCC pairs.
    • Observed elevated expression of beta-actin and glyceraldehyde 3-phosphate dehydrogenase (G3PDH) in HCC tissues.

    Conclusions:

    • cDNA microarray analysis is an effective method for detecting differential gene expression in HCC.
    • The findings contribute to understanding the molecular basis of HCC.
    • Results align with previous studies using different gene expression analysis techniques.