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Updated: Sep 21, 2026

Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy
Published on: May 27, 2018
A faster way to characterize by triple-quantum-filtered (17)O NMR water molecules strongly bound to macromolecules in
A Lehoux1, M Krzystyniak, E Baguet
1Laboratoire d'analyse isotopique et électrochimique de métabolismes, UPRES-A CNRS 6006, Université de Nantes, 2 rue de la Houssinière, Nantes cedex 03, F-44322, France.
Abstract:
The simultaneous use of transverse and longitudinal relaxation rates, together with a transverse triple-quantum-filtering NMR sequence, was estimated for the adequate characterization of (17)O-water relaxation behavior in protein solutions. A complementary contribution to transverse relaxation was found, which was interpreted as chemical exchange of (17)O-water between different sites of the proteins. This contribution was estimated via calibration measurements. Then, for other similar samples, faster experiments could be performed. The analysis of the results obtained in this way gave adequate values of the relaxation rate of water in fast motion, of the fraction of water in slow motion, and of its correlation time. Hence, it permitted the complete characterization of the sample in a reasonable experimental time.
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