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Adiabatic TOCSY MAS in liquids.

E Kupce1, P A Keifer, M Delepierre

  • 1Varian Inc., 28 Manor Road, Walton-on Thames, Oxford, KT12 2QF, United Kingdom. eriks.kupce@varianinc.com

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|January 3, 2001
PubMed
Summary

Magic angle spinning (MAS) disrupts conventional liquid NMR mixing sequences due to field modulations. Adiabatic sequences and rotational resonance conditions offer superior performance in Total Correlation Spectroscopy MAS experiments.

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Area of Science:

  • Nuclear Magnetic Resonance (NMR) Spectroscopy
  • Physical Chemistry
  • Spectroscopic Techniques

Background:

  • Magic Angle Spinning (MAS) is crucial for high-resolution NMR of liquids and solids.
  • Isotropic mixing sequences are vital for coherence transfer in NMR experiments.
  • MAS in liquids can introduce artifacts affecting conventional NMR sequences.

Purpose of the Study:

  • To investigate the impact of MAS on isotropic mixing sequences in liquids.
  • To compare the performance of conventional versus adiabatic mixing sequences under MAS.
  • To identify optimal conditions for Total Correlation Spectroscopy (TOCSY) MAS experiments.

Main Methods:

  • Investigated various isotropic mixing sequences under MAS conditions.
  • Analyzed the effect of RF and magnetic-field inhomogeneities on coherence transfer.

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  • Evaluated performance using Total Correlation Spectroscopy (TOCSY) experiments.
  • Examined performance under rotational resonance conditions.

    • Main Results:

    • MAS in liquids modulates the effective field, compromising conventional mixing sequences.
    • Supercycles amplify performance degradation caused by MAS-induced modulations.
    • Adiabatic mixing sequences demonstrate superior resilience to these modulations.
    • Optimal TOCSY MAS performance was achieved under rotational resonance.

    Conclusions:

    • Conventional isotropic mixing sequences are sensitive to MAS-induced field modulations in liquids.
    • Adiabatic sequences offer a robust alternative for TOCSY MAS experiments.
    • Rotational resonance conditions provide the best performance for TOCSY MAS by minimizing dynamic effects.