Magnetic bead capture eliminates PCR inhibitors in samples collected from the airborne environment, permitting
N Maher1, H K Dillon, S H Vermund
1Division of Geographic Medicine and Department of Environmental Health Sciences, University of Alabama at Birmingham, 35294-2170, USA.
Applied and Environmental Microbiology
|January 3, 2001
Abstract:
PCR detection methods are useful in studies of organisms not amenable to culture. Inhibitors in environmental samples can interfere with such assays. We describe a magnetic bead DNA capture protocol that removes inhibitors from outdoor air samples, maintaining the sensitivity of a 16S Pneumocystis carinii mitochondrial rRNA gene-based PCR.
