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Related Concept Videos

The Cell Cycle Control System02:11

The Cell Cycle Control System

The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
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Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
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Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients
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Cell cycle programs of gene expression control morphogenetic protein localization.

M Lord1, M C Yang, M Mischke

  • 1Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.

The Journal of Cell Biology
|January 3, 2001
PubMed
Summary

Periodic gene expression is crucial for cell division. In yeast, BUD10 gene

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Area of Science:

  • Cell Biology
  • Genetics
  • Molecular Biology

Background:

  • Cell cycle-regulated gene expression is common in yeast, but its functional significance is often unexamined.
  • Many cell cycle-regulated genes are vital for cellular morphogenesis, the process of cell shape determination and formation.

Purpose of the Study:

  • To investigate the importance of cell cycle periodic gene expression in yeast morphogenesis.
  • To analyze the roles of BUD3 and BUD10, two cell cycle-regulated genes essential for axial budding patterns.

Main Methods:

  • Utilized promoter-swap experiments to alter the expression timing of BUD3 and BUD10.
  • Observed the consequences of altered gene expression on protein localization and cellular function.

Main Results:

  • Altering BUD3 expression timing to S/G2 phase affected Bud3p localization timing but not its function.
  • A G1 phase pulse of BUD10 expression was critical for Bud10p localization and function.
  • Bud10p, a membrane protein, requires G1-specific polarized secretion for proper delivery.

Conclusions:

  • Cell cycle-regulated gene expression, particularly a G1 pulse for BUD10, is critical for proper protein localization and function in yeast.
  • Secretion-based targeting of membrane proteins offers cellular flexibility in architecture and evolution.