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Cell cycle programs of gene expression control morphogenetic protein localization.

M Lord¹, M C Yang, M Mischke

¹Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.

The Journal of Cell Biology

|January 3, 2001

Summary

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Periodic gene expression is crucial for cell division. In yeast, BUD10 gene

Area of Science:

Cell Biology
Genetics
Molecular Biology

Background:

Cell cycle-regulated gene expression is common in yeast, but its functional significance is often unexamined.
Many cell cycle-regulated genes are vital for cellular morphogenesis, the process of cell shape determination and formation.

Purpose of the Study:

To investigate the importance of cell cycle periodic gene expression in yeast morphogenesis.
To analyze the roles of BUD3 and BUD10, two cell cycle-regulated genes essential for axial budding patterns.

Main Methods:

Utilized promoter-swap experiments to alter the expression timing of BUD3 and BUD10.
Observed the consequences of altered gene expression on protein localization and cellular function.

Main Results:

Related Experiment Videos

Altering BUD3 expression timing to S/G2 phase affected Bud3p localization timing but not its function.
A G1 phase pulse of BUD10 expression was critical for Bud10p localization and function.
Bud10p, a membrane protein, requires G1-specific polarized secretion for proper delivery.

Conclusions:

Cell cycle-regulated gene expression, particularly a G1 pulse for BUD10, is critical for proper protein localization and function in yeast.
Secretion-based targeting of membrane proteins offers cellular flexibility in architecture and evolution.