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Recombinant expression of lymphocytic choriomeningitis virus strain WE glycoproteins: a single amino acid makes the
W R Beyer1, H Miletic, W Ostertag
1Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie an der Universität Hamburg, D-20251 Hamburg, Germany.
Journal of Virology
|January 3, 2001
Abstract:
Cytoplasmic vector systems are generally used for expression of lymphocytic choriomeningitis virus (LCMV) proteins. However, we achieved high levels of cell surface glycoproteins using a standard nuclear expression plasmid. Expression was independent of other LCMV proteins but was blocked by a missense mutation within the original LCMV(WE) glycoprotein cDNA.
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