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Updated: Jul 12, 2026

Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells
Published on: September 28, 2018
Low-cost microbioreactor for high-throughput bioprocessing
Y Kostov1, P Harms, L Randers-Eichhorn
1Department of Chemical and Biochemical Engineering, University of Maryland Baltimore County, Baltimore, Maryland 21250, USA.
Abstract:
The design of a microbioreactor is described. An optical sensing system was used for continuous measurements of pH, dissolved oxygen, and optical density in a 2 mL working volume. The K(L)a of the microbioreactor was evaluated under different conditions. An Escherichia coli fermentation in both the microbioreactor and a standard 1 L bioreactor showed similar pH, dissolved oxygen, and optical density profiles.%The low cost of the microbioreactor, detection system, and the small volume of the fermentation broth provide a basis for development of a multiple-bioreactor system for high-throughput bioprocess optimization.
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