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Related Experiment Videos

Low-cost microbioreactor for high-throughput bioprocessing.

Y Kostov1, P Harms, L Randers-Eichhorn

  • 1Department of Chemical and Biochemical Engineering, University of Maryland Baltimore County, Baltimore, Maryland 21250, USA.

Biotechnology and Bioengineering
|January 3, 2001
PubMed
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A novel microbioreactor with continuous optical sensing for pH, dissolved oxygen, and optical density was developed. This low-cost system enables high-throughput bioprocess optimization using small fermentation volumes.

Area of Science:

  • Biotechnology
  • Biochemical Engineering

Background:

  • Bioreactor design is critical for bioprocess development and optimization.
  • High-throughput screening requires miniaturized systems with accurate monitoring.
  • Existing systems may be costly or lack integrated real-time analytics.

Purpose of the Study:

  • To describe the design and performance of a novel microbioreactor.
  • To evaluate its capability for continuous monitoring of key fermentation parameters.
  • To assess its suitability for high-throughput bioprocess optimization.

Main Methods:

  • A 2 mL working volume microbioreactor was designed and constructed.
  • An integrated optical sensing system was employed for real-time measurement of pH, dissolved oxygen, and optical density.

Related Experiment Videos

  • The oxygen transfer coefficient (K(L)a) was determined under various operating conditions.
  • Escherichia coli fermentation was conducted and compared between the microbioreactor and a standard 1 L bioreactor.

    • Main Results:

    • The microbioreactor successfully performed continuous measurements of pH, dissolved oxygen, and optical density.
    • Similar fermentation profiles (pH, dissolved oxygen, optical density) were observed when comparing the microbioreactor to a 1 L bioreactor using Escherichia coli.
    • The K(L)a was evaluated, demonstrating the system's performance characteristics.

    Conclusions:

    • The developed microbioreactor offers a low-cost, high-throughput platform for bioprocess optimization.
    • Its small working volume and integrated sensing capabilities facilitate efficient screening.
    • The system demonstrates comparable performance to larger bioreactors, validating its potential for scale-up studies.