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Updated: Oct 10, 2026

Generation of Human Monocyte-derived Dendritic Cells from Whole Blood
Published on: December 24, 2016
[Antigen presenting role of dendritic cell proliferated from peripheral blood monocytes in patients with chronic
1Viral Hepatitis Institute of Chongqing University of Medical Sciences, Chongqing 400010, China.
Objective:
To study the presenting effect of dendritic cell (DC) proliferated from peripheral blood monocytes in patients with chronic hepatitis B on HBsAg.
Methods:
DC proliferated from patient's peripheral blood monocytes by adding GM-CSF and IL-4 were incubated with HBsAg for 1.5h at different concentrations, then mixed with autologous T cell and cocultured in 96-well flat-bottomed microtiter plates for 5 d at 37 degrees C and 5% CO(2). (3)H-TdR was added 13h before the culture completion, and finally the cells were gathered and the cpm was tested.
Results:
The proliferation of T cell by DC treated by HBsAg is significantly higher than untreated.
Conclusion:
DC cultured from peripheral blood monocytes in patients with chronic hepatitis B plays a role in presenting HBsAg.
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