Related Experiment Video

Updated: Aug 12, 2026

Implantation of Fibrin Gel on Mouse Lung to Study Lung-specific Angiogenesis
07:52

Implantation of Fibrin Gel on Mouse Lung to Study Lung-specific Angiogenesis

Published on: December 21, 2014

Plasminogen activators, matrix metalloproteinases and their inhibitors in implanted vascular prostheses

H Urayama1, S Katada, F Kasashima

  • 1First Department of Surgery, Kanazawa University School of Medicine, Kanazawa, Ishikawa, Japan.

European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery
|January 4, 2001
PubMed
Abstract

Insights

Plasminogen activators (PAs) and matrix metalloproteinases (MMPs) are involved in prosthetic graft healing. Lower levels of tissue inhibitor of metalloproteinase-1 (TIMP-1) may contribute to anastomotic aneurysm formation.

Area of Science:

  • Biomedical engineering
  • Vascular surgery
  • Wound healing research

Background:

  • Prosthetic grafts are crucial for vascular reconstruction.
  • Understanding graft healing mechanisms is vital for improving patient outcomes.
  • Plasminogen activators (PAs) and matrix metalloproteinases (MMPs) are key enzymes in tissue remodeling.

Purpose of the Study:

  • To investigate the expression and role of PAs and MMPs in the healing process of explanted vascular grafts.
  • To correlate the presence of these enzymes and their inhibitors with graft characteristics and clinical outcomes.

Main Methods:

  • Immunohistochemical analysis of 30 explanted grafts (Dacron and PTFE).
  • Antibodies used against PAs, MMPs, and their inhibitors (TIMP-1, TIMP-2).
  • Quantification of positive multinucleated giant cells (MGCs) and correlation with implantation duration, lesion type, graft material, and graft status.

Main Results:

  • All grafts expressed PAs and MMPs.
  • No significant differences in MGCs positive for PAs, MMPs, or TIMP-2 were observed between groups.
  • A significantly lower percentage of TIMP-1 was found in grafts with aneurysms compared to those with stenosis.

Conclusions:

  • PAs and MMPs are expressed during vascular prosthesis healing, contributing to cell migration, proliferation, and matrix construction.
  • Under-expression of TIMP-1 is potentially linked to the development of anastomotic aneurysms.
  • These findings offer insights into the molecular mechanisms of graft healing and complications.

More Related Videos

Micropatterning and Assembly of 3D Microvessels13:05

Micropatterning and Assembly of 3D Microvessels

Published on: September 9, 2016

Bacterial Expression and Purification of Human Matrix Metalloproteinase-3 using Affinity Chromatography07:32

Bacterial Expression and Purification of Human Matrix Metalloproteinase-3 using Affinity Chromatography

Published on: March 30, 2022

Related Concept Videos

Role of Matrix Metalloproteases in Degradation of ECM01:23

Role of Matrix Metalloproteases in Degradation of ECM

Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult body.
A...
Anticoagulant Drugs: Low-Molecular-Weight Heparins01:30

Anticoagulant Drugs: Low-Molecular-Weight Heparins

Hemostasis is a crucial process that prevents excessive blood loss from damaged blood vessels. It involves various mechanisms such as vasoconstriction, platelet adhesion and activation, and fibrin formation. The importance of each mechanism depends on the type of vessel injury. In contrast, thrombosis is the abnormal formation of a blood clot within the blood vessels, leading to potential complications if the clot obstructs blood flow. Thrombosis can be caused by increased coagulability of the...
Vascular Spasm01:16

Vascular Spasm

The vascular phase, also known as vasospasm, is the initial stage of hemostasis, crucial for preventing excessive bleeding when a blood vessel is injured. After a vessel is cut, nerves in the damaged area trigger pain and other sensory impulses. Simultaneously, the smooth muscles in the vessel wall contract, resulting in a vascular spasm. This contraction reduces the vessel's diameter at the injury site, slowing or stopping blood loss through the vessel wall. Vascular spasms typically last for...
Formation of the Platelet Plug01:22

Formation of the Platelet Plug

The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
Clot Retraction and Fibrinolysis01:16

Clot Retraction and Fibrinolysis

After a fibrin clot is formed, the next step is clot retraction, a vital process facilitated by platelet contractile proteins, such as actin and myosin. These proteins pull the fibrin strands closer together and condense the clot. This action reduces the size of the clot, creating a smaller, denser structure that effectively seals off the damaged vessel. Clot retraction consolidates the clot and helps with wound healing by bringing the edges of the damaged blood vessel closer together.
Venous Thrombosis III: Interprofessional Care01:29

Venous Thrombosis III: Interprofessional Care

Venous thrombosis requires effective prevention and treatment strategies to improve patient outcomes and reduce potential complications.Prevention StrategiesHealthcare providers must prioritize preventing venous thromboembolism (VTE) for all adult patients upon admission. Interventions depend on bleeding and thrombosis risk, medical history, current medications, diagnoses, planned procedures, and patient preferences. Patients on bed rest should change positions every two hours and, if not...