Related Experiment Videos
Binary typing of Staphylococcus aureus strains through reversed hybridization using digoxigenin-universal linkage
W van Leeuwen1, C Libregts, M Schalk
1Department of Medical Microbiology & Infectious Diseases, Erasmus University Medical Center Rotterdam, 3015 GD Rotterdam, The Netherlands. vanleeuwen@bacl.azr.nl
Journal of Clinical Microbiology
|January 4, 2001
Abstract:
A novel binary typing (BT) procedure, based on reversed hybridization of digoxigenin-universal linkage system-labeled bacterial DNA to strip-immobilized probes, is presented. Chromogenic detection of hybrids was performed. Staphylococcus aureus isolates (n = 20) were analyzed to establish the feasibility of BT. A technically simple and fast procedure has been developed for application in routine microbiology laboratories.