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Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses
Published on: May 2, 2019
Single-tube single-enzyme reverse transcriptase PCR assay for detection of bovine viral diarrhea virus in pooled
D Weinstock1, B Bhudevi, A E Castro
1Animal Diagnostic Laboratory, Department of Veterinary Science, Pennsylvania State University, University Park, Pennsylvania 16802, USA. dxw31@psu.edu
Abstract:
A reverse transcriptase PCR (RT-PCR) was developed for use as a diagnostic screening test for the detection of bovine viral diarrhea virus (BVDV) in pooled bovine serum samples. Individual serum samples from 60 dairy cattle herds located in Pennsylvania were evaluated by the microplate virus isolation method, and pooled sera were analyzed by RT-PCR. RT-PCR was sensitive and specific and detected a single viremic serum sample in up to 100 pooled serum samples. RT-PCR analysis of pooled sera provides a rapid and cost-effective method for the screening of cattle herds for the presence of animals persistently infected with BVDV.
