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Isolation of Legionella anisa using an amoebic coculture procedure
B La Scola1, L Mezi, P J Weiller
1Unité des Rickettsies, CNRS UPRESA 6020, Faculté de Médecine, 13385 Marseille Cedex 05, France.
Journal of Clinical Microbiology
|January 4, 2001
Abstract:
Conventional diagnostic tests for legionellosis were negative for a 61-year-old immunocompromised man with pneumonia. However, coculture of a sputum sample with Acanthamoeba polyphaga amoebae led to the recovery of Legionella anisa. This procedure may be a sensitive and convenient diagnostic method, especially for non-Legionella pneumophila species infections that can be diagnosed only by culture.