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Salmonella vaccines secreting measles virus epitopes induce protective immune responses against measles virus

S Spreng1, I Gentschev, W Goebel

  • 1Lehrstuhl für Mikrobiologie, Theodor-Boveri-Institut für Biowissenschaften, Am Hubland, D-97074, Würzburg, Germany. simone@biozentrum.uni-wuerzburg.de

Microbes and Infection
|January 4, 2001
PubMed

Insights

This study developed a live vaccine using attenuated Salmonella typhimurium to deliver measles virus (MV) antigens. Oral vaccination in mice induced immune responses and offered partial protection against MV encephalitis.

Area of Science:

  • * Vaccinology and immunology
  • * Microbial genetics and molecular biology

Background:

  • * Measles virus (MV) infection poses a significant global health challenge, necessitating effective vaccine strategies.
  • * Current vaccines are effective but require cold chain maintenance and administration by healthcare professionals.
  • * Development of novel, orally administered live vaccines could improve accessibility and efficacy.

Purpose of the Study:

  • * To engineer an attenuated Salmonella typhimurium strain to secrete key measles virus antigens.
  • * To evaluate the immunogenicity and protective efficacy of this novel vaccine candidate in a mouse model.

Main Methods:

  • * Construction of recombinant Salmonella typhimurium aroA (SL7207) secreting MV B-cell and T-cell epitopes using the E. coli alpha-hemolysin secretion system.
  • * Oral immunization of C3H mice with single or combined recombinant S. typhimurium strains.
  • * Assessment of MV-specific humoral and cellular immune responses.
  • * Evaluation of protection against lethal MV encephalitis following intracerebral challenge.

Main Results:

  • * Recombinant S. typhimurium successfully secreted MV antigens, eliciting both humoral and cellular MV-specific immune responses in immunized mice.
  • * Oral vaccination with a combination of the recombinant strains conferred partial protection against a neurotropic MV strain challenge.
  • * The vaccine candidate demonstrated potential for inducing protective immunity against measles virus.

Conclusions:

  • * Attenuated Salmonella typhimurium secreting MV antigens represents a promising platform for a live, orally administered measles vaccine.
  • * The developed vaccine candidate induced measurable immune responses and partial protection, warranting further investigation.
  • * This approach may offer a more accessible and potentially more effective strategy for measles prevention.

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