Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Contryphans from Conus textile venom ducts.

E C Jimenez1, M Watkins, L J Juszczak

  • 1Department of Biology, University of Utah, Salt Lake City, UT 84112, USA.

Toxicon : Official Journal of the International Society on Toxinology
|January 4, 2001
PubMed
Summary

Researchers identified new contryphans, unusual Conus peptides with D-amino acids, from the Conus textile snail. These peptides, including contryphan-Tx and Leu-contryphan-Tx, suggest two major contryphan family branches.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparison of outcomes in dogs undergoing hiatal hernia repair with and without use of a gastropexy: 41 cases (2012-2022).

The Journal of small animal practice·2024
Same author

Nanoghosts: Mesenchymal Stem cells derived nanoparticles as a unique approach for cartilage regeneration.

Journal of controlled release : official journal of the Controlled Release Society·2021
Same author

Effect of bioactive compounds extracted from <i>Cordyceps nidus</i> ANDES-F1080 on laccase activity of <i>Pleurotus ostreatus</i> ANDES-F515.

Biotechnology reports (Amsterdam, Netherlands)·2020
Same author

Co-delivery of immunomodulators in biodegradable nanoparticles improves therapeutic efficacy of cancer vaccines.

Biomaterials·2019
Same author

pH-responsive poly(lactide-co-glycolide) nanoparticles containing near-infrared dye for visualization and hyaluronic acid for treatment of osteoarthritis.

Journal of controlled release : official journal of the Controlled Release Society·2019
Same author

A heterodyne dispersion interferometer for wide bandwidth density measurements on DIII-D.

The Review of scientific instruments·2018

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Pharmacology

Background:

  • Contryphans are a unique class of Conus peptides characterized by post-translational modifications, specifically the incorporation of D-tryptophan or D-leucine.
  • Cone snail venom composition and function are areas of active research due to the diverse bioactive peptides they produce.

Purpose of the Study:

  • To identify and characterize novel contryphans from the cone snail Conus textile.
  • To synthesize and analyze the properties of newly discovered contryphan peptides.
  • To investigate the structural and functional implications of D-amino acid incorporation in Conus peptides.

Main Methods:

  • cDNA cloning to identify contryphan precursors.
  • Peptide synthesis of inferred mature contryphan sequences.

Related Experiment Videos

  • Reverse-phase high-performance liquid chromatography (RP-HPLC) for peptide separation.
  • Ultraviolet resonance Raman spectroscopy (UVRRS) for structural analysis.

    • Main Results:

    • Identification and synthesis of three new contryphans: contryphan-Tx, Leu-contryphan-Tx, and contryphan R/Tx.
    • Contryphan-Tx exhibited two distinct peaks in RP-HPLC, indicating conformational heterogeneity.
    • UVRRS revealed differences in the D-tryptophan dihedral angle between contryphan-Tx conformers.
    • Peptide sequences and in vivo effects suggest two distinct evolutionary branches within the contryphan family.

    Conclusions:

    • The study expands the known diversity of contryphans by identifying new variants from Conus textile.
    • Structural analysis reveals conformational flexibility in contryphan-Tx, influenced by the D-tryptophan residue.
    • The findings support a model of two major evolutionary lineages for contryphan peptides.