Diagnosis of granulocytic ehrlichiosis in humans by immunofluorescence assay

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P Brouqui1, E Salvo, J S Dumler

  • 1Unité des rickettsies, WHO Collaborative Center for Rickettsial Reference and Research, CNRS UPRES A 6020, Faculté de Médecine, 13385 Marseille Cedex 5 France. Philippe.Brouqui@medecine.univ-mrs.fr

Clinical and Diagnostic Laboratory Immunology
|January 4, 2001
PubMed

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Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
The...